TY - JOUR T1 - Selective Enhancement of [<sup>3</sup>H]Opiate Agonist Binding by Divalent Cations JF - Molecular Pharmacology JO - Mol Pharmacol SP - 735 LP - 744 VL - 11 IS - 6 AU - GAVRIL W. PASTERNAK AU - ADELE M. SNOWMAN AU - SOLOMON H. SNYDER Y1 - 1975/11/01 UR - http://molpharm.aspetjournals.org/content/11/6/735.abstract N2 - Manganese ions enhance binding of the tritiated opiate agonists dihydromorphine and levorphanol 78% and 64%, respectively, while binding of the tritiated antagonists naloxone, levallorphan, and diprenorphine is unaffected. Magnesium and nickel ions also selectively enhance [3H]dihydromorphine binding with no effect on [3H]naloxone binding. By contrast, cupric and ferrous ions lower the binding of [3H]dihydromorphine far more than that of [3H]naloxone. Manganese ions also enhance the ability of unlabeled agonists to inhibit the binding of [3H]naloxone. All these effects are most pronounced in the presence of sodium chloride. Ethylenediaminetetracetic acid inhibits the binding of [3H]dihydromorphine 50% without altering [3H] naloxone binding, while ethylenebis[(oxyethylenenitrilo)]tetraacetic acid and magnesium and manganese complexes of EDTA are ineffective. The EDTA effect can be reversed by subsequent addition of either manganese or magnesium ions. The divalent cations which enhance opiate agonist binding appear to counteract the ability of sodium ions to inhibit agonist binding selectively. The potency of sodium in inhibiting [3H]dihydromorphine binding is reduced 5-fold by the addition of manganese ions. There is a good correlation between the sensitivity of opiate receptor binding to sodium and manganese. ACKNOWLEDGMENT We thank Mr. Marty Katz for his contribution to the initial phases of this study. ER -