TY - JOUR T1 - The Stimulation of Cathecholamine Release from Chromaffin Granules by Valinomycin JF - Molecular Pharmacology JO - Mol Pharmacol SP - 745 LP - 750 VL - 11 IS - 6 AU - JACQUELINE DOLAIS-KITABGI AU - ROBERT L. PERLMAN Y1 - 1975/11/01 UR - http://molpharm.aspetjournals.org/content/11/6/745.abstract N2 - Valinomycin stimulates the release of catecholamine from isolated guinea pig adrenal medullary chromaffin granules incubated in vitro. Valinomycin-stimulated catecholamine release is dependent on the ionophore concentration and on the concentration of K+ in the incubation medium. In. 0.15 M KCl-5 mM Na·PO4, pH 7.0, valinomycin at 1 µM increases catecholamine release about 5-fold. The finding that catecholamine release is accompained by the release of adenine nucleotides and of dopamine β-hydroxylase from the chromaffin granules indicates that valinomycin induces the lysis of the granules. The effect of valinomycin on catecholamine release is also dependent upon the anions present in the incubation medium. The anions tested support valinomycin-stimulated catecholamine release in the order SCN-, I-, Br- > Cl- > acetate, F-, isethionate. These data suggest that, in the presence of a membrane-permeable anion, valinomycin-mediated transport of K+ into chromaffin granules leads to the osmotic lysis of these granules. ACKNOWLEDGMENT We are grateful to Ms. Michi Chen for technical assistance. ER -