RT Journal Article
SR Electronic
T1 Reductive Activation and Inactivation of Mitomycin As Studied with Human and Bacterial Cell Cultures
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 202
OP 204
VO 1
IS 2
A1 WACLAW SZYBALSKI
A1 V. G. ARNESON
YR 1965
UL http://molpharm.aspetjournals.org/content/1/2/202.abstract
AB Sodium borohydride at noninhibitory concentrations (50 µg/ml) reverses almost completely the lethal effects of mitomycin C when added within 5 min (human cell cultures) or 10 sec (bacteria) after cell exposure to the antibiotic. Sodium cyanide, dinitrophenol, and phenethyl alcohol, shown previously to augment the bactericidal effects of mitomycin, have no influence on the lethal action of this antibiotic for human cells. ACKNOWLEDGMENTS We are indebted to Dr. S. Wakaki (Kyowa Hakko Kogyo Co., Tokyo) for supplying us with mitomycin C, and to Dr. Elizabeth H. Szybalska for her editorial help. This study was supported by Grant CA-07175 from the National Cancer Institute.