PT  - JOURNAL ARTICLE
AU  - DANIEL T. MINKEL
AU  - CHRISTINE CHAN-STIER
AU  - DAVID H. PETERING
TI  - Reactions of 3-Ethoxy-2-oxobutyraldehyde Bis(<em>N</em><sup>4</sup>-dimethylthiosemicarbazonato)-Zinc(II) with Tumor Cells and Mitochondria
DP  - 1976 Nov 01
TA  - Molecular Pharmacology
PG  - 1036--1044
VI  - 12
IP  - 6
4099  - http://molpharm.aspetjournals.org/content/12/6/1036.short
4100  - http://molpharm.aspetjournals.org/content/12/6/1036.full
SO  - Mol Pharmacol1976 Nov 01; 12
AB  - Physicochemical and biological properties of bis(thiosemicarbazonato)-zinc complexes have been investigated. Respiration of Ehrlich ascites cells is inhibited by 3-ethoxy-2-oxobutyraldehyde bis(N4-dimethylthiosemicarbazonato)-zinc(II). Zinc ion is much less effective, but the thiosemicarbazone ligand initially stimulates, then depresses, oxygen uptake. State 3 oxygen uptake of tumor cell mitochondria is more sensitive to this zinc complex than is state 4. In bovine heart mitochondria respiratory inhibition occurs almost exclusively in state 3. The insensitivity of the electron transport chain to the complex was confirmed with Keilin-Hartree particles. Formation constants and partition coefficients for several bis(thiosemicarbazonato)-zinc complexes are used to interpret the different behavior of zinc ion, thiosemicarbazone ligand, and zinc complex with tumor cells and mitochondria.