PT  - JOURNAL ARTICLE
AU  - S. K. ARYA
AU  - R. CHAWDA
TI  - Polyribonucleotide Inhibition of Murine Leukemia Virus Replication: Effect of Strandedness
DP  - 1977 Mar 01
TA  - Molecular Pharmacology
PG  - 374--377
VI  - 13
IP  - 2
4099  - http://molpharm.aspetjournals.org/content/13/2/374.short
4100  - http://molpharm.aspetjournals.org/content/13/2/374.full
SO  - Mol Pharmacol1977 Mar 01; 13
AB  - We have previously reported on the structure-activity relationships of polyribonucleotides as inhibitors of murine leukemia virus replication in cultured cells. These studies raised the possibility that the active form of otherwise single-stranded polyribonucleotides may be a multistranded structure. We have now compared the inhibitory potencies of double- and triple-stranded complexes of poly(adenylic acid) and poly(uridylic acid) with those of single-stranded poly(adenylic acid) and poly(uridylic acid). We find that poly(adenylic acid) · poly(uridylic acid) and poly(adenylic acid). 2 poly(uridylic acid) are considerably less potent than uncomplexed poly(adenylic acid) and poly(uridylic acid). These results suggest that the inhibitory potential of polyribonucleotides does not reside in a multistranded structure but in a single-stranded molecule. These observations are consistent with the notion that the inhibition of RNA tumor virus replication by single-stranded polyribonucleotides is more likely to be due to their effect on reverse transcription than on interferon induction.