PT - JOURNAL ARTICLE AU - LEONARD M. HJELMELAND AU - LEWIS ARONOW AU - JAMES R. TRUDELL TI - Intramolecular Determination of Substituent Effects in Hydroxylations Catalyzed by Cytochrome P-450 DP - 1977 Jul 01 TA - Molecular Pharmacology PG - 634--639 VI - 13 IP - 4 4099 - http://molpharm.aspetjournals.org/content/13/4/634.short 4100 - http://molpharm.aspetjournals.org/content/13/4/634.full SO - Mol Pharmacol1977 Jul 01; 13 AB - Hydroxylation of monosubstituted 1,3-diphenylpropanes by rat liver microsomes leads predominantly to a mixture of the isomeric benzylic alcohols at positions 1 and 3. Quantitation of the relative concentrations of these alcohols can be achieved by combined gas chromatography-mass fragmentography. p-Methyl, p-trifluoromethyl, and p-fluoro substituents yield product distributions which clearly indicate an electrophilic mechanism for the hydroxylation reaction. ACKNOWLEDGMENTS The authors gratefully acknowledge the expert assistance of Dr. Elaine Herron and the Finnigan Corporation in obtaining mass fragmentograms.