TY - JOUR T1 - Incorporation of 2-Fluoro-L-histidine into Cellular Protein JF - Molecular Pharmacology JO - Mol Pharmacol SP - 1105 LP - 1110 VL - 13 IS - 6 AU - DAVID C. KLEIN AU - JOAN L. WELLER AU - KENNETH L. KIRK AU - ROBERT W. HARTLEY Y1 - 1977/11/01 UR - http://molpharm.aspetjournals.org/content/13/6/1105.abstract N2 - The purpose of this study was to determine whether mammalian cells can utilize 2-fluoro-L-histidine in protein synthesis. Rat pineal glands were incubated in organ culture in medium containing 2-fluoro-L-[4-3]histidine (3 mM); trichloracetic acid-insoluble material from these glands contained radioactivity. Incorporation of radioactivity was decreased in the presence of histidine (3 mM). After the 3H-labeled macromolecules were treated with a protease, over 75% of the radioactivity was soluble in trichloracetic acid. Amino acid analysis of the trichloracetic acid-soluble material indicated that the majority of the liberated radioactivity was 2-fluoro-L-[3H]histidine. These findings demonstrate that mammalian cells can incorporate 2-fluoro-L-histidine into newly synthesized proteins. In view of this, it would appear likely that the reported inhibitory effects of 2-fluoro-L-histidine on enzyme induction could result, in part, from the incorporation of the analogue into proteins. ER -