RT Journal Article
SR Electronic
T1 Incorporation of 2-Fluoro-L-histidine into Cellular Protein
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 1105
OP 1110
VO 13
IS 6
A1 DAVID C. KLEIN
A1 JOAN L. WELLER
A1 KENNETH L. KIRK
A1 ROBERT W. HARTLEY
YR 1977
UL http://molpharm.aspetjournals.org/content/13/6/1105.abstract
AB The purpose of this study was to determine whether mammalian cells can utilize 2-fluoro-L-histidine in protein synthesis. Rat pineal glands were incubated in organ culture in medium containing 2-fluoro-L-[4-3]histidine (3 mM); trichloracetic acid-insoluble material from these glands contained radioactivity. Incorporation of radioactivity was decreased in the presence of histidine (3 mM). After the 3H-labeled macromolecules were treated with a protease, over 75% of the radioactivity was soluble in trichloracetic acid. Amino acid analysis of the trichloracetic acid-soluble material indicated that the majority of the liberated radioactivity was 2-fluoro-L-[3H]histidine. These findings demonstrate that mammalian cells can incorporate 2-fluoro-L-histidine into newly synthesized proteins. In view of this, it would appear likely that the reported inhibitory effects of 2-fluoro-L-histidine on enzyme induction could result, in part, from the incorporation of the analogue into proteins.