PT  - JOURNAL ARTICLE
AU  - M. GRANT
AU  - D. R. PHILLIPS
TI  - Dissociation of Polydeoxynucleotide-Daunomycin Complexes
DP  - 1979 Jul 01
TA  - Molecular Pharmacology
PG  - 357--360
VI  - 16
IP  - 1
4099  - http://molpharm.aspetjournals.org/content/16/1/357.short
4100  - http://molpharm.aspetjournals.org/content/16/1/357.full
SO  - Mol Pharmacol1979 Jul 01; 16
AB  - The first order rate constants for the dissociation of daunomycin from polydeoxynucleotides have been measured at 20° and 37°. The rate constants at a given temperature were similar and were in the order poly(dA)·poly(dT) > poly(dG)·poly(dC) > poly(dA-dT)· poly(dA-dT) > poly(dG-dC)·poly(dG-dC) at both temperatures. A comparison of these data with inhibition studies of DNA polymerase (using the same polydeoxynucleotide templates) indicates that at the DNA level, the mechanism of action of daunomycin is primarily due to disruption of the DNA secondary structure (i.e., dependent on the amount of daunomycin bound to the DNA template) but is not directly dependent on the rate of release of daunomycin from the DNA. ACKNOWLEDGMENTS The technical assistance of Miss Gayle Carlyle is acknowledged, as is the gift of daunomycin from Farmitalia, Milan.