TY - JOUR T1 - Adenylate Cyclase in Cerebral Microvessels: Action of Guanine Nucleotides, Adenosine, and other Agonists JF - Molecular Pharmacology JO - Mol Pharmacol SP - 462 LP - 472 VL - 16 IS - 2 AU - MINTA HUANG AU - GEORGE I. DRUMMOND Y1 - 1979/09/01 UR - http://molpharm.aspetjournals.org/content/16/2/462.abstract N2 - The isolation of microvessels (capillaries and arterioles) from guinea pig cerebral cortex is described. Adenylate cyclase in microvessel preparations was activated by guanylylimidodiphosphate in a time-dependent manner. The rate of guanyl nucleotide activation was enhanced by adenosine, 2-chloroadenosine, 2-azidoadenosine, norepinephrine, histamine, isoproterenol, prostaglandin E1, prostaglandin E2 and prostacyclin. Activation by adenosine analogues was demonstrable only when endogenously-generated adenosine was destroyed with adenosine deaminase. Activation was also in evidence when microvessels were incubated with agonists together with guanine nucleotide, then washed by sedimentation prior to assay, reflecting persistence of the activated state. ER -