RT Journal Article
SR Electronic
T1 The Incorporation of Trifluorothymidine into Calf Thymus DNA in a Cell-Free System Does Not Lead to Chain Termination
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 148
OP 150
VO 18
IS 1
A1 AWNI M. SARRIF
A1 HIROSHI TONE
A1 PETER V. DANENBERG
A1 CHARLES HEIDELBERGER
YR 1980
UL http://molpharm.aspetjournals.org/content/18/1/148.abstract
AB In this report we present evidence that trifluorothymidine-5'-triphosphate (F3dTTP) once incorporated into activated calf thymus DNA by a partially purified HeLa cell cytoplasmic DNA-dependent DNA polymerase α does not lead to chain termination. Less than 1% of the [3H]F3dTTP incorporated in the cell-free system was recovered at the 3'-termini. In addition, F3dTTP-containing DNA was capable of incorporating [3H]dTTP, which led to the complete disappearance of radioactive trifluorothymidine from the 3'-termini. ACKNOWLEDGMENTS The authors acknowledge Ms. Virginia Garcia for her technical assistance and Mrs. Sharon Garrison for the preparation of the manuscript.