RT Journal Article
SR Electronic
T1 Adenosine Receptor-Mediated Inhibition of Rat Cerebral Cortical Adenylate Cyclase by a GTP-Dependent Process
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 598
OP 601
VO 18
IS 3
A1 DERMOT M. F. COOPER
A1 CONSTANTINE LONDOS
A1 MARTIN RODBELL
YR 1980
UL http://molpharm.aspetjournals.org/content/18/3/598.abstract
AB Adenosine analogues inhibit rat cerebral cortical adenylate cyclase by a receptor-mediated GTP-dependent process. N6-Phenylisopropyladenosine and other analogues of adenosine are potent inhibitors (Ki values of 10-100 nM) of adenylate cyclase in a particulate preparation of rat cerebral cortex. The detection of such inhibitory effects is facilitated by the use of a deoxy ATP assay system. The inhibition is strictly dependent on GTP, is amplified by Na+ ions, and is antagonized by methylxanthines. ACKNOWLEDGMENTS The authors wish to thank Drs. John Daly and Bill Padgett for helpful discussions and advice on membrane preparations. The technical assistance of Mr. James Oden and the expert secretarial assistance of Ms. Bonnie Richards are also gratefully acknowledged.