RT Journal Article
SR Electronic
T1 The formation of diarachidonyl diglyceride by rat neutrophils.
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 688
OP 693
VO 21
IS 3
A1 M I Siegel
A1 R T McConnell
A1 D A Brent
A1 P Chandrasurin
A1 R D Macfarlane
A1 C J McNeal
YR 1982
UL http://molpharm.aspetjournals.org/content/21/3/688.abstract
AB Neutrophils isolated from the pleural cavity of rats 3 hr after the intrapleural injection of carrageenan metabolize exogenously added arachidonic acid via cyclooxygenase and lipoxygenase. In addition, these cells esterify arachidonic acid to produce diarachidonyl diglyceride. The structure of the diglyceride was determined with the use of various chemical and enzymatic digestions, gas chromatography-mass spectrometry, and 252Cf plasma-desorption mass spectrometry. The formation of this unique diglyceride is stimulated by the presence of nonsteroidal anti-inflammatory drugs. Some of the possible consequences of diarachidonyl diglyceride production are discussed.