RT Journal Article
SR Electronic
T1 A Study of Hepatic Carbonic Anhydrase
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 144
OP 157
VO 2
IS 2
A1 THOMAS H. MAREN
A1 ARTHUR C. ELLISON
A1 SUSAN K. FELLNER
A1 WALTER B. GRAHAM
YR 1966
UL http://molpharm.aspetjournals.org/content/2/2/144.abstract
AB Dog and rat liver contain carbonic anhydrase in supernatant and particulate fractions. Hepatic bile of both species has higher HC03- and lower Cl- concentration than plasma. The enzyme in all fractions of dog liver was inhibited by unsubstituted aromatic sulfonamides at concentrations of 10-6 to 10-9 M. In vivo, administration of these drugs at doses which yield > 99% enzyme inhibition lowered biliary HCO3- and raised biliary Cl- concentrations. Certain of the drugs were secreted by liver and concentrated in the bile of both species. Rat liver supernatant carbonic anhydrase was nearly refractory to sulfonamide inhibition in vitro; this is the first example of such lowered response in the animal kingdom. Biliary electrolytes in the rat were accordingly not altered by high doses of these sulfonamides.