PT - JOURNAL ARTICLE AU - R I Glazer AU - K D Hartman TI - In vitro translational activity of messenger RNA following treatment of human colon carcinoma cells with sangivamycin. DP - 1983 Nov 01 TA - Molecular Pharmacology PG - 509--512 VI - 24 IP - 3 4099 - http://molpharm.aspetjournals.org/content/24/3/509.short 4100 - http://molpharm.aspetjournals.org/content/24/3/509.full SO - Mol Pharmacol1983 Nov 01; 24 AB - Total mRNA from human colon carcinoma cell line HT-29 treated with the pyrrolopyrimidine antibiotic, sangivamycin(7-deaza-7-carboxamidoadenosine), was assessed in vitro using a reticulocyte lysate translation system. Under conditions of known drug-induced cell lethality, sangivamycin-modified mRNA showed a diminished translational capacity. The decreased activity of drug-modified mRNA increased in proportion to the time-dependent cytocidal effects of sangivamycin in this cell line. These data suggest that the lethal effects of this drug may be associated, in part, with a reduced ability of drug-modified mRNA to sustain translation in situ.