RT Journal Article
SR Electronic
T1 In vitro translational activity of messenger RNA following treatment of human colon carcinoma cells with sangivamycin.
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 509
OP 512
VO 24
IS 3
A1 R I Glazer
A1 K D Hartman
YR 1983
UL http://molpharm.aspetjournals.org/content/24/3/509.abstract
AB Total mRNA from human colon carcinoma cell line HT-29 treated with the pyrrolopyrimidine antibiotic, sangivamycin(7-deaza-7-carboxamidoadenosine), was assessed in vitro using a reticulocyte lysate translation system. Under conditions of known drug-induced cell lethality, sangivamycin-modified mRNA showed a diminished translational capacity. The decreased activity of drug-modified mRNA increased in proportion to the time-dependent cytocidal effects of sangivamycin in this cell line. These data suggest that the lethal effects of this drug may be associated, in part, with a reduced ability of drug-modified mRNA to sustain translation in situ.