RT Journal Article
SR Electronic
T1 The Mechanism of the Inhibition of Aminotransferases by Salicylate
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 526
OP 533
VO 2
IS 6
A1 B. J. GOULD
A1 P. D. DAWKINS
A1 M. J. H. SMITH
A1 A. J. LAWRENCE
YR 1966
UL http://molpharm.aspetjournals.org/content/2/6/526.abstract
AB Salicylate inhibits pig heart alanine and aspartate aminotransferase activities in vitro. The inhibitions are reversible, involving competition with α-oxoglutarate and alanine for the alanine aminotransferase and with α-oxoglutarate and aspartate for the aspartate aminotransferase. The results are consistent with a binary mechanism for the action of the aminotransferases. In addition they show that salicylate inhibits alanine aminotransferase much more strongly than aspartate aminotransferase. These results are discussed with reference to some of the in vivo actions of the drug. ACKNOWLEDGMENT We wish to thank the Arthritis and Rheumatism Research Council for generous financial support and the Nuffield Foundation for a grant towards the purchase of the spectrophotometer used in the work.