@article {Gyenes719, author = {M Gyenes and M Farrant and D H Farb}, title = {"Run-down" of gamma-aminobutyric acidA receptor function during whole-cell recording: a possible role for phosphorylation.}, volume = {34}, number = {6}, pages = {719--723}, year = {1988}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {When using whole-cell recording methods and a minimal intracellular medium containing only inorganic ions, ethyleneglycolbis-(beta-aminoethyl ether)-N,N,N{\textquoteright},N{\textquoteright}-tetraacetic acid, and N-2-hydroxyethylpiperazine-N{\textquoteright}-2-ethanesulfonic acid, we have observed a time-dependent decrease in the responsiveness of cultured chick spinal cord neurons to gamma-aminobutyric acid (GABA). The current evoked by 30 microM GABA progressively declined to approximately 30\% of its initial value after five applications at 10-min intervals. This was accompanied by an equivalent decline in the GABA-evoked membrane conductance. "Run-down" of the response was reduced when Mg2+-ATP was present in the pipet solution. Inclusion of ATP-gamma-S, an analog that donates a thiophosphate group resistant to hydrolysis, also reduced run-down. The nonhydrolyzable analog beta, gamma-imidoadenosine-5{\textquoteright}-triphosphate was without effect. These results suggest that an ATP-dependent process, possibly phosphorylation, is involved in the maintenance of GABAA receptor function.}, issn = {0026-895X}, URL = {https://molpharm.aspetjournals.org/content/34/6/719}, eprint = {https://molpharm.aspetjournals.org/content/34/6/719.full.pdf}, journal = {Molecular Pharmacology} }