PT  - JOURNAL ARTICLE
AU  - KUN, ERNEST
AU  - VOLFIN, PIERRE
AU  - LOH, HORACE H.
TI  - Studies with Specific Enzyme Inhibitors
DP  - 1967 Nov 01
TA  - Molecular Pharmacology
PG  - 497--502
VI  - 3
IP  - 6
4099  - http://molpharm.aspetjournals.org/content/3/6/497.short
4100  - http://molpharm.aspetjournals.org/content/3/6/497.full
SO  - Mol Pharmacol1967 Nov 01; 3
AB  - Rates of O2 uptake of mitochondria vary depending on the nature of added substrate, on assay conditions, and on the tissue from which mitochondria are isolated. Inhibition of aconitase by fluorocitrate influenced O2 uptake and citrate accumulation in a highly selective manner. Metabolic effectivity of fluorocitrate was dependent on the same variables which determine specific metabolic rates in the absence of the inhibitor. It is proposed that the experimental design applied detects metabolic consequences of tissue-specific control mechanisms. ACKNOWLEDGMENTS The work reported in this paper was supported by research grants of the U.S. Public Health Service (RO1-HD-01239-11 and RO1-CA-07955-03), the National Science Foundation (GB-5749), and the American Heart Association, Inc. (66-652).