RT Journal Article
SR Electronic
T1 Studies with Specific Enzyme Inhibitors
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 497
OP 502
VO 3
IS 6
A1 KUN, ERNEST
A1 VOLFIN, PIERRE
A1 LOH, HORACE H.
YR 1967
UL http://molpharm.aspetjournals.org/content/3/6/497.abstract
AB Rates of O2 uptake of mitochondria vary depending on the nature of added substrate, on assay conditions, and on the tissue from which mitochondria are isolated. Inhibition of aconitase by fluorocitrate influenced O2 uptake and citrate accumulation in a highly selective manner. Metabolic effectivity of fluorocitrate was dependent on the same variables which determine specific metabolic rates in the absence of the inhibitor. It is proposed that the experimental design applied detects metabolic consequences of tissue-specific control mechanisms. ACKNOWLEDGMENTS The work reported in this paper was supported by research grants of the U.S. Public Health Service (RO1-HD-01239-11 and RO1-CA-07955-03), the National Science Foundation (GB-5749), and the American Heart Association, Inc. (66-652).