@article {Datta435, author = {R Datta and S Kharbanda and D Kufe}, title = {Regulation of jun-B gene expression by 1-beta-D-arabinofuranosyl-cytosine in human myeloid leukemia cells.}, volume = {38}, number = {4}, pages = {435--439}, year = {1990}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {The jun-B gene is a member of the jun family of immediate early response genes that regulate cellular responses to growth factors. The present studies have examined the effects of 1-beta-D-arabinofuranosylcytosine (ara-C) on jun-B expression in human KG-1 myeloid leukemia cells. The results demonstrate that ara-C increases jun-B mRNA levels. The level of jun-B transcripts was maximal after 12 hr of exposure to 10(-5) M ara-C and persisted through 72 hr. Nuclear run-on assays demonstrated that ara-C treatment is associated with an increased rate of jun-B gene transcription. The results also demonstrate that ara-C-induced jun-B mRNA levels are regulated by a posttranscriptional mechanism. The level of jun-B transcripts in ara-C-treated cells was superinduced by inhibition of protein synthesis. Moreover, cycloheximide prolonged the half-life of ara-C-induced jun-B transcripts. These results, thus, demonstrate that ara-C induces expression of the jun-B gene in KG-1 cells and that this effect is mediated by transcriptional and posttranscriptional mechanisms.}, issn = {0026-895X}, URL = {https://molpharm.aspetjournals.org/content/38/4/435}, eprint = {https://molpharm.aspetjournals.org/content/38/4/435.full.pdf}, journal = {Molecular Pharmacology} }