PT  - JOURNAL ARTICLE
AU  - M A Proll
AU  - R B Clark
TI  - Potent Gi-mediated inhibition of adenylyl cyclase by a phosphonate analog of monooleylphosphatidate.
DP  - 1991 Jun 01
TA  - Molecular Pharmacology
PG  - 740--744
VI  - 39
IP  - 6
4099  - http://molpharm.aspetjournals.org/content/39/6/740.short
4100  - http://molpharm.aspetjournals.org/content/39/6/740.full
SO  - Mol Pharmacol1991 Jun 01; 39
AB  - We have previously demonstrated that monooleylphosphatidate (MOPA) and phosphatidate inhibit adenylyl cyclase in cultured fibroblasts. In this study, the specificity of the phospholipid effect was probed by analysis of the effect of phosphonate analogs of these phospholipids on adenylyl cyclase in C6 glioma cells. The MOPA phosphonate analog inhibited adenylyl cyclase, but the comparable phosphonate analog of phosphatidate was ineffective. The IC50 for inhibition of adenylyl cyclase by the MOPA phosphonate analog was similar to that of MOPA, the maximal inhibitions were comparable (approximately 45% inhibition of hormone-stimulated adenylyl cyclase), and the effects of both appeared to be mediated by Gi, because treatment with islet-activating protein reduced the inhibition to 5-10%.