@article {Lummis18, author = {S C Lummis and I L Martin}, title = {Solubilization, purification, and functional reconstitution of 5-hydroxytryptamine3 receptors from N1E-115 neuroblastoma cells.}, volume = {41}, number = {1}, pages = {18--23}, year = {1992}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {5-Hydroxytryptamine3 (5-HT3) receptors from N1E-115 neuroblastoma cells were solubilized using 1.1\% n-octylglucoside; five other detergents were less effective. Purification was achieved by affinity chromatography using immobilized GR119566X and biospecific elution with quipazine. Saturation analyses with [3H] GR67330 binding revealed high affinity binding to homogeneous populations of sites in both the solubilized (Kd = 0.05 +/- 0.02 nM) and purified (Kd = 0.10 +/- 0.04 nM) preparations. Competition experiments indicated that the solubilized and purified receptor preparations retained the characteristics observed in N1E-115 cells in vivo. Polyacrylamide gel electrophoresis of the purified receptor revealed a single protein band of 54.7 +/- 1.3 kDa. The purified receptor was incorporated into liposomes, and the functional integrity of the protein was demonstrated by measurement of m-chlorophenylbiguanide-stimulated 22Na uptake. Saturation analysis of the reconstituted preparation revealed a Kd of 0.24 +/- 0.07 nM and suggested that 0.2\% of 5-HT3 receptors present in the original membrane preparation had been incorporated into liposomes.}, issn = {0026-895X}, URL = {https://molpharm.aspetjournals.org/content/41/1/18}, eprint = {https://molpharm.aspetjournals.org/content/41/1/18.full.pdf}, journal = {Molecular Pharmacology} }