RT Journal Article
SR Electronic
T1 Cross-species comparison of 5-lipoxygenase-activating protein.
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 1014
OP 1019
VO 42
IS 6
A1 P J Vickers
A1 G P O'Neill
A1 J A Mancini
A1 S Charleson
A1 M Abramovitz
YR 1992
UL http://molpharm.aspetjournals.org/content/42/6/1014.abstract
AB To identify regions of 5-lipoxygenase-activating protein (FLAP) important for the function of the protein and the binding of leukotriene biosynthesis inhibitors, we performed a cross-species analysis of FLAP. FLAP from all 10 mammalian species analyzed (human, monkey, horse, pig, cow, sheep, rabbit, dog, rat, and mouse) were immunologically cross-reactive and specifically bound leukotriene biosynthesis inhibitors with high affinity. Using the polymerase chain reaction, cDNA clones for FLAP from six species (monkey, horse, pig, sheep, rabbit, and mouse) were isolated and sequenced. The deduced amino acid sequences of FLAP show a high degree of identity to each other and to the published sequences for human and rat FLAP. Two regions of the protein are almost totally conserved among all of the species analyzed. This suggests that these regions have functional significance and may be involved in inhibitor binding.