PT  - JOURNAL ARTICLE
AU  - SHEEN, M. R.
AU  - KIM, BYUNG KYU
AU  - PARKS, R. E.
TI  - Purine Nucleoside Phosphorylase from Human Erythrocytes III. Inhibition by the Inosine Analog Formycin B of the Isolated Enzyme and of Nucleoside Metabolism in Intact Erythrocytes and Sarcoma 180 Cells
DP  - 1968 May 01
TA  - Molecular Pharmacology
PG  - 293--299
VI  - 4
IP  - 3
4099  - http://molpharm.aspetjournals.org/content/4/3/293.short
4100  - http://molpharm.aspetjournals.org/content/4/3/293.full
SO  - Mol Pharmacol1968 May 01; 4
AB  - Formycin B, an analog of inosine, is a potent competitive inhibitor of purified erythrocytic purine nucleoside phosphorylase. The inhibition constant, Ki (1 x 10-4 M) is of the same order of magnitude as the Km for inosine (5 x 10-5 M). Formycin B also inhibits the degradation of nucleosides by intact and hemolyzed human erythrocytes and by Sarcoma 180 ascites cells. ACKNOWLEDGMENT The authors would like to express their appreciation to Dr. Sungnan Cha for his help in the preparation of this manuscript.