RT Journal Article
SR Electronic
T1 The Sequential Increase in the Rates of Synthesis of Enzymes in Rat Liver after Glucocorticoid Administration
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 168
OP 172
VO 4
IS 2
A1 YEE S. KIM
YR 1968
UL http://molpharm.aspetjournals.org/content/4/2/168.abstract
AB The relative rate of synthesis of glutamic-alanine transaminase (GAT) in rat liver was measured at various times after the administration of massive doses of prednisolone sodium phosphate or hydrocortisone succinate by the L-leucine-14C pulse-labeling method. The rate of synthesis was unchanged 3.5 hr after the administration of prednisolone and 6.5 hr after the administration of hydrocortisone. However, the rate was increased approximately 2-fold after 6.5 hr of prednisolone administration, and approximately 3-fold after 2 days of prednisolone or 5 days of hydrocortisone. Thus, the first noticeable increase in the GAT synthesis, occurring some 6 hr after the hormone administration, lags behind those of glutamic-tyrosine transaminase (GTT) and tryptophan pyrrolase (TP) which have been shown to occur within 3 hr after an administration of hormone. This observation indicates that the stimulatory effect of glucocorticoid hormone on the synthesis of liver proteins are sequential rather than simultaneous. ACKNOWLEDGMENT This work was supported by Research Grant AM 11280-01 National Institute of Arthritis and Metabolic Diseases U.S. Public Health Service.