PT  - JOURNAL ARTICLE
AU  - Blaxall, H S
AU  - Cerutis, D R
AU  - Hass, N A
AU  - Iversen, L J
AU  - Bylund, D B
TI  - Cloning and expression of the alpha 2C-adrenergic receptor from the OK cell line.
DP  - 1994 Feb 01
TA  - Molecular Pharmacology
PG  - 176--181
VI  - 45
IP  - 2
4099  - http://molpharm.aspetjournals.org/content/45/2/176.short
4100  - http://molpharm.aspetjournals.org/content/45/2/176.full
SO  - Mol Pharmacol1994 Feb 01; 45
AB  - The alpha 2-adrenergic receptors have been divided into four pharmacological subtypes, alpha 2A, alpha 2B, alpha 2C, and alpha 2D. The OK cell line, a cell line derived from an opossum kidney, expresses the alpha 2C-adrenergic receptor and is the prototypical cell line for the alpha 2C receptor subtype. The cloned human alpha 2C-C4 and rat RG10 receptors have been shown to express alpha 2C pharmacology. Here we report the cloning and expression of the OK alpha 2C-adrenergic receptor, OKc2. The receptor has 64% deduced amino acid identity and 21% similarity to the alpha 2-C4 receptor, giving an overall similarity of 85%. The clone, expressed in Chinese hamster ovary cells, has a pharmacology that correlates very well (r = 0.97) with that of the native OK cell alpha 2C-adrenergic receptor, and it is negatively coupled to adenylyl cyclase.