PT  - JOURNAL ARTICLE
AU  - S Grissmer
AU  - A N Nguyen
AU  - J Aiyar
AU  - D C Hanson
AU  - R J Mather
AU  - G A Gutman
AU  - M J Karmilowicz
AU  - D D Auperin
AU  - K G Chandy
TI  - Pharmacological characterization of five cloned voltage-gated K+ channels, types Kv1.1, 1.2, 1.3, 1.5, and 3.1, stably expressed in mammalian cell lines.
DP  - 1994 Jun 01
TA  - Molecular Pharmacology
PG  - 1227--1234
VI  - 45
IP  - 6
4099  - http://molpharm.aspetjournals.org/content/45/6/1227.short
4100  - http://molpharm.aspetjournals.org/content/45/6/1227.full
SO  - Mol Pharmacol1994 Jun 01; 45
AB  - We have analyzed the biophysical and pharmacological properties of five cloned K+ (Kv) channels (Kv1.1, Kv1.2, Kv1.3, Kv1.5, and Kv3.1) stably expressed in mammalian cell lines. Kv1.1 is biophysically similar to a K+ channel in C6 glioma cells and astrocytes, Kv1.3 and Kv3.1 have electrophysiological properties identical to those of the types n and l K+ channels in T cells, respectively, and Kv1.5 closely resembles a rapidly activating delayed rectifier in the heart. Each of these native channels may be formed from the homomultimeric association of the corresponding Kv subunits, and pharmacological compounds that selectively modulate them may be useful for the treatment of neurological, immune, and cardiac disorders. The cell lines described in this report could be used to identify such drugs and we have therefore embarked on a pharmacological characterization of the five cloned channels. The compounds tested in this study include 4-aminopyridine, capsaicin, charybdotoxin, cromakalim, dendrotoxin, diltiazem, D-sotalol, flecainide, kaliotoxin, mast cell degranulating peptide, nifedipine, noxiustoxin, resiniferatoxin, and tetraethylammonium.