TY - JOUR T1 - c-myc Down-regulation in suramin-treated HL60 cells precedes growth inhibition but does not trigger differentiation. JF - Molecular Pharmacology JO - Mol Pharmacol SP - 73 LP - 78 VL - 46 IS - 1 AU - N Ben-Baruch AU - W C Reinhold AU - N Alexandrova AU - I Ichinose AU - M Blake AU - J B Trepel AU - M Zajac-Kaye Y1 - 1994/07/01 UR - http://molpharm.aspetjournals.org/content/46/1/73.abstract N2 - Down-regulation of c-myc mRNA expression is linked to growth arrest and the state of differentiation of hematopoietic cells. We showed that treatment of HL60 cells with suramin results in a rapid reduction of c-myc expression followed by an inhibition of cell growth. Both c-myc mRNA and protein levels decreased by day 1 of treatment, and, by day 4, only 10% of control c-myc protein levels were detected. In contrast to retinoic acid, dimethyl sulfoxide, and 12-O-tetradecanoylphorbol-13-acetate treatment, however, exposure of HL60 cells to suramin did not result in the induction of differentiation. These results demonstrate that suramin modulates c-myc levels in HL60 cells and that the down-regulation of c-myc is not sufficient to trigger differentiation toward either granulocytic or monocytic lineages. ER -