RT Journal Article
SR Electronic
T1 c-myc Down-regulation in suramin-treated HL60 cells precedes growth inhibition but does not trigger differentiation.
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 73
OP 78
VO 46
IS 1
A1 N Ben-Baruch
A1 W C Reinhold
A1 N Alexandrova
A1 I Ichinose
A1 M Blake
A1 J B Trepel
A1 M Zajac-Kaye
YR 1994
UL http://molpharm.aspetjournals.org/content/46/1/73.abstract
AB Down-regulation of c-myc mRNA expression is linked to growth arrest and the state of differentiation of hematopoietic cells. We showed that treatment of HL60 cells with suramin results in a rapid reduction of c-myc expression followed by an inhibition of cell growth. Both c-myc mRNA and protein levels decreased by day 1 of treatment, and, by day 4, only 10% of control c-myc protein levels were detected. In contrast to retinoic acid, dimethyl sulfoxide, and 12-O-tetradecanoylphorbol-13-acetate treatment, however, exposure of HL60 cells to suramin did not result in the induction of differentiation. These results demonstrate that suramin modulates c-myc levels in HL60 cells and that the down-regulation of c-myc is not sufficient to trigger differentiation toward either granulocytic or monocytic lineages.