TY - JOUR T1 - Studies on Serum Cholinesterase Kinetics by Nuclear Magnetic Resonance Spectroscopy JF - Molecular Pharmacology JO - Mol Pharmacol SP - 640 LP - 644 VL - 4 IS - 6 AU - G. KATO Y1 - 1968/11/01 UR - http://molpharm.aspetjournals.org/content/4/6/640.abstract N2 - The hydrolysis of acetylcholine by horse serum cholinesterase has been measured by nuclear magnetic resonance spectroscopy. The reaction rate was estimated from the formation of free acetate, whose characteristic frequency of absorption differs from that of the acetate moiety of acetylcholine. From the decay of the acetate signal of acetylcholine and/or the formation of the sodium acetate signal in sodium phosphate buffer, it is possible to follow the rate of the reaction. The calculated Km for the substrate and the Ki for various inhibitors agree well with values obtained by others using conventional methods. ACKNOWLEDGMENT I wish to thank Professor K. Krnjević for his advice and for reading the manuscript. ER -