PT  - JOURNAL ARTICLE
AU  - C M Boeve
AU  - A V Wyngaerd
AU  - M De Ley
TI  - Inhibition of human interferon-gamma biosynthesis by an antisense RNA-expressing vector.
DP  - 1996 Jan 01
TA  - Molecular Pharmacology
PG  - 58--62
VI  - 49
IP  - 1
4099  - http://molpharm.aspetjournals.org/content/49/1/58.short
4100  - http://molpharm.aspetjournals.org/content/49/1/58.full
SO  - Mol Pharmacol1996 Jan 01; 49
AB  - We applied antisense RNA technology for reducing the level of human IFN-gamma (HuIFN-gamma) expression. An antisense RNA vector containing the full-length HuIFN-gamma cDNA in the opposite orientation was electroporated into cells constitutively producing very high levels of the cytokine. Approximately 53% of the resulting clones exhibited a specific HuIFN-gamma inhibition of an average of 95.5%. The results of reverse transcription-polymerase chain reaction and Northern blot analyses revealed that the antisense effect originated from a specific reduction of the targeted mRNA caused by antisense RNA expression. This very effective antisense RNA strategy can have possible therapeutic applications in treating diseases where HuIFN-gamma is known to play a negative role, such as in certain autoimmune diseases.