PT  - JOURNAL ARTICLE
AU  - J Kapur
AU  - R L Macdonald
TI  - Pharmacological properties of gamma-aminobutyric acidA receptors from acutely dissociated rat dentate granule cells.
DP  - 1996 Sep 01
TA  - Molecular Pharmacology
PG  - 458--466
VI  - 50
IP  - 3
4099  - http://molpharm.aspetjournals.org/content/50/3/458.short
4100  - http://molpharm.aspetjournals.org/content/50/3/458.full
SO  - Mol Pharmacol1996 Sep 01; 50
AB  - The pharmacological properties of gamma-aminobutyric acid (GABA) type A receptor (GABAR) currents recorded from hippocampal dentate granule cells acutely dissociated from 28-35-day-old rats were characterized using the whole-cell patch-clamp technique. Granule cells were voltage-clamped to 0 mV, and GABA was applied using a modified U-tube rapid-application technique. All granule cells were moderately sensitive to GABA (EC50 = 47 microM). All granule cell GABAR currents were uniformly sensitive to Zn2+ (IC50 = 29 microM), diazepam (EC50 = 158 nM), zolpidem (EC50 = 75 nM), and dimethoxyl-4-ethyl-beta-carboline-3-carboxylate (IC50 = 60 nM). GABAR currents from only 50% of granule cells were sensitive to loreclezole (EC50 = 9 microM). These data suggest that hippocampal dentate granule cells expressed GABARs with distinctive pharmacological properties of two types: loreclezole-sensitive and -insensitive receptors. It is likely that these distinctive properties were due to the specific GABAR subtypes that assembled to produce distinct granule cell GABAR isoforms.