TY - JOUR T1 - Coexpression with potassium channel subunits used to clone the Y2 receptor for neuropeptide Y. JF - Molecular Pharmacology JO - Mol Pharmacol SP - 387 LP - 390 VL - 49 IS - 3 AU - J M Rimland AU - E P Seward AU - Y Humbert AU - E Ratti AU - D G Trist AU - R A North Y1 - 1996/03/01 UR - http://molpharm.aspetjournals.org/content/49/3/387.abstract N2 - Xenopus oocytes were injected with RNAs for the two inward-rectifier potassium channel subunits Kir3.1 (GIRK1) and Kir3.4 (rcKATP or CIR) in addition to RNA from the neuroblastoma cell line KAN-TS. Potassium currents were evoked by neuropeptide Y in oocytes injected with polyadenylated RNA or with cRNA from pools of a neuroblastoma (KAN-TS) cDNA library, and progressive subdivision of responding pools yielded a single cDNA. The encoded protein contains 381 amino acids, has the seven hydrophobic domains characteristic of G protein-coupled receptors, and is 31% identical to the Y1 receptor: potassium currents were induced by neuropeptide Y (EC50=60pm) and Y2-selective analogues. Coexpression with potassium channel subunits will be a generally useful method for the cloning of G protein-coupled receptors. ER -