PT  - JOURNAL ARTICLE
AU  - J. M. TRIFARÓ
TI  - The Effect of Ca<sup>++</sup> Omission on the Secretion of Catecholamines and the Incorporation of Orthophosphate-<sup>32</sup>P into Nucleotides and Phospholipids of Bovine Adrenal Medulla during Acetylcholine Stimulation
DP  - 1969 Jul 01
TA  - Molecular Pharmacology
PG  - 424--427
VI  - 5
IP  - 4
4099  - http://molpharm.aspetjournals.org/content/5/4/424.short
4100  - http://molpharm.aspetjournals.org/content/5/4/424.full
SO  - Mol Pharmacol1969 Jul 01; 5
AB  - Bovine adrenal medullary slices were incubated at 30° in Locke’s solution containing orthophosphate-32P (50 µCi/ml) with and without Ca++, and were stimulated with acetylcholine (10-5 M) in the presence of eserine (10-5 M). The omission of Ca++, as expected, abolished the increment in catecholamine secretion due to acetylcholine stimulation. Acetylcholine stimulation increased the incorporation of 32P into phospholipids, particularly into phosphatidic acid and into phosphatidylinositol, in both the presence and absence of Ca++. Thus, a lack of correlation between catecholamine release and 32P incorporation into phospholipids upon acetylcholine stimulation was observed. ACKNOWLEDGMENTS I wish to thank Dr. A. Tenenhouse for reading the manuscript. Highly competent technical assistance was rendered by Mr. S. Iu.