RT Journal Article
SR Electronic
T1 Antagonistic Action of Anti-androgens on the Formation of a Specific Dihydrotestosterone-Receptor Protein Complex in Rat Ventral Prostate
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 428
OP 431
VO 5
IS 4
A1 SENMAW FANG
A1 SHUTSUNG LIAO
YR 1969
UL http://molpharm.aspetjournals.org/content/5/4/428.abstract
AB Cyproterone (1,2α-methylene-6-chloro-Δ4,6-pregnadien-17α-ol-3,20-dione) 17α-acetate, a potent anti-androgen, suppressed the uptake of radioactive androgens in vivo by the ventral prostate of rats. This was accompanied by a decrease in the retention of 5α-dihydrotestosterone (17β-hydroxy-5α-androstan-3-one) by prostate cell nuclei . Cyproterone and its 17α-acetate (less than 0.5 µg/ml) also inhibited the formation of a specific dihydrotestosterone-protein complex in prostate cell nuclei when minced prostate was incubated with radioactive testosterone or dihydrotestosterone. Estradiol-17β, diethylstilbestrol, and progesterone, but not hydrocortisone succinate, also suppressed the retention of dihydrotestosterone by prostatic cell nuclei in vitro, but to a much lesser extent than cyproterone. ACKNOWLEDGMENTS The authors wish to express their appreciation to Mrs. A. H. Lin, Mrs. D. Sagher, and Mr. J. L. Tymoczko for their skillful technical assistance.