@article {Gordon554, author = {Adrienne S. Gordon and Lina Yao and Zhi-Liang Wu and Imogen R. Coe and Ivan Diamond}, title = {Ethanol Alters the Subcellular Localization of δ- and ε Protein Kinase C in NG108{\textendash}15 Cells}, volume = {52}, number = {4}, pages = {554--559}, year = {1997}, doi = {10.1124/mol.52.4.554}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Protein kinase C (PKC) has been shown to regulate the ethanol sensitivity of membrane-bound receptors and transporters, but little is known about the molecular mechanisms underlying this regulation. PKC is a family of isozymes that translocate to new intracellular sites on activation. Here we present immunochemical data showing that ethanol causes translocation of δ- and ε-PKC to new intracellular sites. Ethanol causes translocation of δ-PKC from the Golgi to the perinucleus; this translocation is similar to that induced by activation of PKC with phorbol esters. In contrast, ε-PKC translocation caused by ethanol is different from that induced by phorbol esters; ethanol causes translocation of ε-PKC from the perinucleus to the cytoplasm, whereas phorbol ester activation causes translocation of ε-PKC to the nucleus. Because the substrate specificity of these kinases is determined by their site of localization, ethanol-induced translocation of δ- and ε-PKC to new intracellular sites may explain some of the pleiotropic effects of ethanol on cellular functions.}, issn = {0026-895X}, URL = {https://molpharm.aspetjournals.org/content/52/4/554}, eprint = {https://molpharm.aspetjournals.org/content/52/4/554.full.pdf}, journal = {Molecular Pharmacology} }