RT Journal Article SR Electronic T1 Selective Regulation of G Protein-Coupled Receptor-Mediated Signaling by G Protein-Coupled Receptor Kinase 2 in FRTL-5 Cells: Analysis of Thyrotropin, α1B-Adrenergic, and A1 Adenosine Receptor-Mediated Responses JF Molecular Pharmacology JO Mol Pharmacol FD American Society for Pharmacology and Experimental Therapeutics SP 316 OP 324 DO 10.1124/mol.56.2.316 VO 56 IS 2 A1 Luisa Iacovelli A1 Rossana Franchetti A1 Deborah Grisolia A1 Antonio De Blasi YR 1999 UL http://molpharm.aspetjournals.org/content/56/2/316.abstract AB G protein-coupled receptor kinases (GRKs) play a key role in the process of receptor homologous desensitization. In the present study, we address the question of whether a variety of receptors coupled to different G protein subtypes and naturally expressed on the same cell are selectively regulated by GRK2. The signaling stimulated by thyrotropin (TSH), α1B-adrenergic, and A1adenosine receptors was studied in FRTL-5 cells permanently transfected to overexpress GRK2 and GRK2-K220R, a kinase dead GRK dominant negative mutant. In FRTL-5 overexpressing GRK2, TSH-induced cyclic AMP response was attenuated, indicating that TSH receptor is desensitized by this kinase. Consistently, FRTL-5 cells overexpressing GRK2-K220R show increased TSH-induced cyclic AMP response, demonstrating that this receptor is under tonic control by GRK. Unlike TSH receptor, α1B-adrenergic receptor response was unaffected in FRTL-5 overexpressing GRK2 and GRK2-K220R. When A1 adenosine receptors were stimulated, Giα-mediated cyclic AMP inhibition was totally unaffected by overexpression of either GRK2 or GRK2-K220R. By contrast, Gβγ-mediated response (activation of mitogen-activated protein kinases) was efficiently desensitized by GRK2 but was unaffected by GRK2-K220R overexpression. The present study documents that overexpression of GRK2 results in a selective regulation of different G protein-coupled receptors expressed on the same cell and that this kinase can regulate preferentially only one of the different pathways activated by the same receptor. The preferential regulation of the A1 adenosine receptor-stimulated mitogen-activated protein kinases by GRK2 indicates that this kinase can have additional regulatory effects on Gβγ-stimulated pathways, possibly through direct binding and regulation of the receptor-Gβγ complex.