TY - JOUR T1 - Expression of α-Transducin in Chinese Hamster Ovary Cells Stably Transfected with the Human δ-Opioid Receptor Attenuates Chronic Opioid Agonist-Induced Adenylyl Cyclase Superactivation JF - Molecular Pharmacology JO - Mol Pharmacol SP - 1076 LP - 1082 DO - 10.1124/mol.60.5.1076 VL - 60 IS - 5 AU - Marc Rubenzik AU - Eva Varga AU - Dagmar Stropova AU - William R. Roeske AU - Henry I. Yamamura Y1 - 2001/11/01 UR - http://molpharm.aspetjournals.org/content/60/5/1076.abstract N2 - To investigate the role of G-protein βγ subunits in δ-opioid signal transduction, we have transfected Chinese hamster ovary (CHO) cells stably expressing the human δ-opioid receptor (hDOR/CHO cells) with the Gα-subunit of transducin-1 (hDOR/T1/CHO). Inhibition of forskolin-stimulated adenylyl cyclase and phospholipase Cβ (PLCβ) activation was measured in each of these cell lines. Because PLCβ3 activation in CHO cells has been shown to be mediated by free Gβγ subunits derived from Gαi/o, the action of transducin was confirmed by measuring a significant attenuation of (+)-4-[(αR)-α-((2S,5R)-4-Allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethylbenzamide (SNC80)-mediated maximal inositol-1,4,5-trisphosphate formation in transducin-expressing cells of 59 ± 12% compared with control cells. The acute inhibition of cAMP formation was unchanged between control and transducin-expressing cells. We show that cells stably expressing the human δ-opioid receptor exhibited a pertussis toxin-sensitive cAMP overshoot in response to chronic application of SNC80. After 4 h of pretreatment and washout with 100 nM SNC80, maximal forskolin-stimulated cAMP formation in hDOR/CHO cells increased by 229 ± 37% compared with buffer-treated cells. Expression of transducin in hDOR/CHO cells diminished this response: hDOR/T1/CHO cells showed no significant change in maximal forskolin-stimulated cAMP formation after pretreatment and washout. These data indicate that the expression of α-transducin scavenges free Gβγsubunits and, furthermore, that free Gβγ subunits play a role in opioid-mediated PLCβ activation and adenylyl cyclase superactivation, but not acute inhibition of forskolin-stimulated cAMP formation in hDOR/CHO cells. ER -