RT Journal Article SR Electronic T1 Inflammation Enhances μ-Opioid Receptor Transcription and Expression in Mice Intestine JF Molecular Pharmacology JO Mol Pharmacol FD American Society for Pharmacology and Experimental Therapeutics SP 894 OP 899 DO 10.1124/mol.60.5.894 VO 60 IS 5 A1 Olga Pol A1 Francesc Alameda A1 Margarita M. Puig YR 2001 UL http://molpharm.aspetjournals.org/content/60/5/894.abstract AB Opioid receptors (ORs) and their mRNA are present in the central and peripheral nervous systems of mammals and in different peripheral tissues, including the gut. Using a model of croton oil-induced (CO) intestinal inflammation in mice, we have shown a 6-fold increase in the potency of the antitransit and antisecretory effects of μ-OR agonists, mediated by peripheral ORs. We postulate that the enhanced effects are mediated by an increase in the expression of intestinal OR. We used jejunum (stripped of the mucosal layer) from mice with CO-induced intestinal inflammation and, as control subjects, saline-treated animals (SS). We evaluated the quantity of μ-OR mRNA determined by a competitive reverse-transcriptase polymerase chain reaction; the levels of μ-OR protein by Western blot immunoassay, and the localization and number of cells expressing μ-OR using immunohistochemistry. The results show a significant increase of μ-OR mRNA (7.7-fold) and receptor protein (3-fold) during intestinal inflammation. Inflammation also induced a 64.3% increase in the number of neurons expressing μ-OR immunoreactivity in the myenteric plexus but not in the submucosal plexus. Our results show that intestinal inflammation enhances the transcription and translation of μ-OR mRNA, thus explaining the increased potency of μ-opioids during inflammation.