PT  - JOURNAL ARTICLE
AU  - JULIUS AXELROD
AU  - ELLIOT S. VESELL
TI  - Heterogeneity of <em>N</em>- and <em>O</em>-Methyltransferases
DP  - 1970 Jan 01
TA  - Molecular Pharmacology
PG  - 78--84
VI  - 6
IP  - 1
4099  - http://molpharm.aspetjournals.org/content/6/1/78.short
4100  - http://molpharm.aspetjournals.org/content/6/1/78.full
SO  - Mol Pharmacol1970 Jan 01; 6
AB  - The methyltransferases phenylethanolamine N-methyltransferase, histamine N-methyltransferase (EC 2.1.1.8), catechol O-methyltransferase (EC 2.1.1.6), and hydroxyindole O-methyltransferase, from tissues of several species, were subjected to starch block electrophoresis. Adrenal phenylethanolamine N-methyltransferase and pineal hydroxyindole O-methyltransferase were heterogeneous among different species with respect to electrophoretic mobility on starch block, heat stability, and substrate specificity, but they were homogeneous within a given species. Multiple forms of histamine N-methyltransferase and catechol O-methyltransferase occurred in tissues within a given species as well as among different species. They had different electrophoretic mobilities, heat stabilities, and kinetic properties. ACKNOWLEDGMENTS We thank Dorothy M. Rutherford and Wallace W. Holland for their excellent technical assistance.