RT Journal Article
SR Electronic
T1 Immunofluorescent Localization of Dopamine β-Hydroxylase in Tissues
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 85
OP 92
VO 6
IS 1
A1 BOYD K. HARTMAN
A1 SIDNEY UDENFRIEND
YR 1970
UL http://molpharm.aspetjournals.org/content/6/1/85.abstract
AB Dopamine β-hydroxylase from bovine adrenal medulla has been prepared in a form free from antigenic contaminants. The purified enzyme was used to induce the production of immunologically pure antienzyme in rabbits. The latter was utilized in the indirect immunofluorescent method for localization of dopamine β-hydroxylase in peripheral tissues and brain. Time cross-reactivity of the antibody to the bovine enzyme permits its application to studies with tissues from laboratory animals.