TY - JOUR T1 - Cyclic Adenosine 3',5'-Monophosphate Formation in Brain Slices: Stimulation by Batrachotoxin, Ouabain, Veratridine, and Potassium Ions JF - Molecular Pharmacology JO - Mol Pharmacol SP - 184 LP - 188 VL - 6 IS - 2 AU - HIROTOSHI SHIMIZU AU - CYRUS R. CREVELING AU - JOHN W. DALY Y1 - 1970/03/01 UR - http://molpharm.aspetjournals.org/content/6/2/184.abstract N2 - The formation of cyclic adenosine 3',5'-monophosphate-14C from endogenous ATP-14C is greatly stimulated on incubation of cerebral cortex slices with depolarizing agents such as batrachotoxin, veratridine, ouabain, and potassium ion. In slices of cerebral cortex, batrachotoxin is the most potent known stimulant (ED50 = 1 x 10-7 M) of cyclic 3',5'-AMP-14C formation. Stimulation of cyclic 3',5'-AMP-14C formation by depolarizing agents requires calcium ions and is inhibited by theophylline. Stimulation of cyclic 3',5'-AMP-14C formation by histamine, in contrast, does not require calcium ions and is not inhibited by theophylline. The results suggest a relationship among depolarization, transmitter release, and cyclic 3',5'-AMP formation in brain. ER -