RT Journal Article
SR Electronic
T1 Effect of 2,2' -(9,1O-Anthrylenedimethylene)bis(2-thiopseudourea) on Nucleic Acid Synthesis in Bacteria
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 335
OP 344
VO 6
IS 4
A1 PRISCILLA P. SAUNDERS
A1 GRADY F. SAUNDERS
YR 1970
UL http://molpharm.aspetjournals.org/content/6/4/335.abstract
AB The mechanism of action of 2,2'-(9,10-anthrylenedimethylene)bis(2-thiopseudourea) (ATPU or pseudourea) has been investigated in bacterial systems. Inhibition of the growth of Bacillus subtilis by ATPU was reversed by the addition of nucleic acids. The synthesis of DNA and RNA in vivo was markedly inhibited by the drug. RNA synthesis in vitro catalyzed by purified DNA-dependent RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) of Bacillus stearothermophilus was sensitive to ATPU in a concentration-dependent manner. This inhibition was reversed by increasing the concentration of DNA primer. Evidence for a drug-DNA interaction was obtained from viscosity and cesium chloride buoyant density studies, which suggested the possible formation of intercalation complexes between DNA and ATPU. ACKNOWLEDGMENTS The authors are indebted to Mrs. V. Liu, Miss G. Schultz, and Miss E. Fortin for excellent technical assistance. We thank Dr. E. Frei, III, for discussiouns on the clinical aspects of ATPU.