@article {ALLEN441, author = {JULIUS C. ALLEN and HENRY R. BESCH, JR. and GERALD GLICK and ARNOLD SCHWARTZ and ROBERT M. LEWIS}, title = {The Binding of Tritiated Ouabain to Sodium- and Potassium-Activated Adenosine Triphosphatase and Cardiac Relaxing System of Perfused Dog Heart}, volume = {6}, number = {4}, pages = {441--443}, year = {1970}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Isolated dog hearts were perfused in situ with ouabain; control hearts were perfused for similar lengths of time without the addition of ouabain. After the onset of a significant positive inotropic effect, the hearts were removed, and (Na+ + K+)-ATPase and cardiac relaxing system were isolated. The (Na+ + K+)-ATPase activity was significantly decreased in the ouabain-treated hearts. The binding in vitro of 3H-ouabain to the (Na+ + K+)-ATPase of the treated hearts was significantly lower than biniding to control enzyme. A small extent of binding of 3H-ouabain to the cardiac relaxing system was observed and may be attributed to the presence of (Na+ + K+)-ATPase.}, issn = {0026-895X}, URL = {https://molpharm.aspetjournals.org/content/6/4/441}, eprint = {https://molpharm.aspetjournals.org/content/6/4/441.full.pdf}, journal = {Molecular Pharmacology} }