RT Journal Article
SR Electronic
T1 Interaction Between the μ Opioid Receptor and Filamin A Is Involved in Receptor Regulation and Trafficking
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 1092
OP 1100
DO 10.1124/mol.64.5.1092
VO 64
IS 5
A1 Onoprishvili, Irma
A1 Andria, Matthew L.
A1 Kramer, Hal K.
A1 Ancevska-Taneva, Natasa
A1 Hiller, Jacob M.
A1 Simon, Eric J.
YR 2003
UL http://molpharm.aspetjournals.org/content/64/5/1092.abstract
AB The carboxyl tail of the human μ opioid receptor was shown to bind the carboxyl terminal region of human filamin A, a protein known to couple membrane proteins to actin. Results from yeast two-hybrid screening were confirmed by direct protein-protein binding and by coimmunoprecipitation of filamin and μ opioid receptor from cell lysates. To investigate the role of filamin A in opioid receptor function and regulation, we used the melanoma cell line M2, which does not express filamin A, and its subclone A7, transfected with human filamin A cDNA. Both cell lines were stably transfected with cDNA encoding myc-tagged human μ opioid receptor. Fluorescent studies, using confocal microscopy, provided evidence that filamin and μ opioid receptors were extensively colocalized on the membranes of filamin-expressing melanoma cells. The immunostaining of μ opioid receptors indicated that the lack of filamin had no detectable effect on membrane localization of the receptors. Moreover, μ opioid receptors function normally in the absence of filamin A, as evidenced by studies of opioid binding and DAMGO inhibition of forskolin-stimulated adenylyl cyclase. However, agonist-induced receptor down-regulation and functional desensitization were virtually abolished in cells lacking filamin A. The level of internalized μ-opioid receptors, after 30-min exposure to agonist, was greatly reduced, suggesting a role for filamin in μ opioid receptor trafficking. During these studies, we observed that forskolin activation of adenylyl cyclase was greatly reduced in filamin-lacking cells. An even more unexpected finding was the ability of long-term treatment with [d-Ala2,N-Me-Phe4,Gly5-ol]-enkephalin of M2 cells, containing μ opioid receptors, to restore normal forskolin activation. The mechanism of this effect is currently unknown. It is postulated that the observed effects on μ opioid receptor regulation by filamin A and, by implication, of the actin cytoskeleton may be the result of its role in μ opioid receptor trafficking.