PT - JOURNAL ARTICLE AU - Geneviève Tavernier AU - Maria Jimenez AU - Jean-Paul Giacobino AU - Nicolas Hulo AU - Max Lafontan AU - Patrick Muzzin AU - Dominique Langin TI - Norepinephrine Induces Lipolysis in β<sub>1</sub>/β<sub>2</sub>/β<sub>3</sub>-Adrenoceptor Knockout Mice AID - 10.1124/mol.105.014670 DP - 2005 Sep 01 TA - Molecular Pharmacology PG - 793--799 VI - 68 IP - 3 4099 - http://molpharm.aspetjournals.org/content/68/3/793.short 4100 - http://molpharm.aspetjournals.org/content/68/3/793.full SO - Mol Pharmacol2005 Sep 01; 68 AB - Catecholamines are major stimulants of adipose tissue metabolism. Norepinephrine and epinephrine act through three subtypes of β-adrenoceptors (β-AR) expressed in the adipocytes. The aim of this work was to study the mechanisms of lipid mobilization in β1/β2/β3-AR triple-knockout (β-less) mice. Glycerol and nonesterified fatty acids released from isolated adipocytes were measured as an index of lipolytic activity. There was no difference between the two genotypes for basal lipolysis and lipolytic response to corticotropin or to agents acting at the adenylyl cyclase and protein kinase A levels. The lipolytic response to norepinephrine and β-AR agonists was blunted in β-less mice. However, a residual low-affinity lipolytic effect was observed in the presence of catecholamines and β3-AR agonists but not of β1- or β2-AR agonists. cAMP levels were increased by a β-AR agonist in white and brown adipocytes of β-less mice. The residual lipolytic effect was blocked by β-AR antagonists. It was mediated neither by α1- or α2-AR nor dopaminergic, serotonergic, and histaminergic by receptors. Bioinformatic analyses do not provide evidence for a fourth β-AR. We conclude that the residual lipolytic effect observed in β-less mice can be attributed to an unknown Gs-protein-coupled receptor with low affinity for catecholamines.