@article {Ghisolfi816, author = {Laura Ghisolfi and Laura Papucci and Annamaria Bevilacqua and Gianfranco Canti and Giuseppe Tataranni and Andrea Lapucci and Nicola Schiavone and Sergio Capaccioli and Angelo Nicolin}, title = {Increased Bcl2 Expression by Antisense Oligoribonucleotides Targeting the Adenine-Uridine-Rich Element Motif}, volume = {68}, number = {3}, pages = {816--821}, year = {2005}, doi = {10.1124/mol.105.014357}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {RNA has become a promising target for pharmacological purposes. Most current strategies are directed toward down-regulating its functions. In this study, we provide evidence of the up-regulation of messenger RNA in a sequence-specific manner. The bcl2 (b)-ARE (adenine-uridine-rich element) in the 3'-untranslated region of the b-RNA that regulates the rate of RNA degradation has been targeted with three chemically modified oligoribonucleotides designed in the antisense orientation (asORNs). The three asORNs were studied by a cell-free degradation assay. All three slowed the rate of RNA decay in a dose-response fashion, they were specific to the b-ARE, and two of them were individually effective. asORNs were then transfected into the malignant cells in culture and b-RNA half-life was measured by real-time reverse transcriptase-polymerase chain reaction. We showed that by stabilizing b-RNA the three asORNs increased the expression of b-RNA and of the relevant protein in a dose-response fashion.}, issn = {0026-895X}, URL = {https://molpharm.aspetjournals.org/content/68/3/816}, eprint = {https://molpharm.aspetjournals.org/content/68/3/816.full.pdf}, journal = {Molecular Pharmacology} }