PT  - JOURNAL ARTICLE
AU  - Gotti, Cecilia
AU  - Moretti, Milena
AU  - Zanardi, Alessio
AU  - Gaimarri, Annalisa
AU  - Champtiaux, Nicolas
AU  - Changeux, Jean-Pierre
AU  - Whiteaker, Paul
AU  - Marks, Michael J.
AU  - Clementi, Francesco
AU  - Zoli, Michele
TI  - Heterogeneity and Selective Targeting of Neuronal Nicotinic Acetylcholine Receptor (nAChR) Subtypes Expressed on Retinal Afferents of the Superior Colliculus and Lateral Geniculate Nucleus: Identification of a New Native nAChR Subtype α3β2(α5 or β3) Enriched in Retinocollicular Afferents
AID  - 10.1124/mol.105.015925
DP  - 2005 Oct 01
TA  - Molecular Pharmacology
PG  - 1162--1171
VI  - 68
IP  - 4
4099  - http://molpharm.aspetjournals.org/content/68/4/1162.short
4100  - http://molpharm.aspetjournals.org/content/68/4/1162.full
SO  - Mol Pharmacol2005 Oct 01; 68
AB  - The activation of neuronal nicotinic acetylcholine receptors (nAChRs) has been implicated in the activity-dependent development and plasticity of retina and the refinement of retinal projections. Pharmacological and functional studies have also indicated that different presynaptic nAChRs can have a modulatory function in retinotectal synapses. We biochemically and pharmacologically identified the multiple nAChR subtypes expressed on retinal afferents of the superior colliculus (SC) and lateral geniculate nucleus (LGN). We found that the α6β2* and α4(nonα6)β2* nAChRs are the major receptor populations expressed in both SC and LGN. In addition, the LGN contains two minor populations of α2α6β2* and α3β2* subtypes, whereas the SC contains a relatively large population of a new native subtype, the α3β2(α5/β3) nAChR. This subtype binds the α-conotoxin MII with an affinity 50 times lower than that of the native α6β2* subtype. Studies of tissues obtained from eye-enucleated animals allowed the identification of nAChRs expressed by retinal afferents: in SC α6β2*, α4α6β2*, and α3β2* (approximately 45, 35, and 20%, respectively), in LGN, α4α6β2*, α6β2*, α4β2*, α2α6β2*, and α3β2* (approximately 40, 30, 20, 5, and 5%, respectively). In both regions, more than 50% of nAChRs were not expressed by retinal afferents and belonged to the α4β2* (90%) or α4α5β2* (10%) subtypes. Moreover, studies of the SC tissues obtained from wild-type and α4, α6, and β3 knockout mice confirmed and extended the data obtained in rat tissue and allowed a comprehensive dissection of the composition of nAChR subtypes present in this retinorecipient area.