TY - JOUR T1 - Analysis of G Protein βγ Dimer Formation in Live Cells Using Multicolor Bimolecular Fluorescence Complementation Demonstrates Preferences of β<sub>1</sub> for Particular γ Subunits JF - Molecular Pharmacology JO - Mol Pharmacol SP - 194 LP - 205 DO - 10.1124/mol.106.022616 VL - 70 IS - 1 AU - Stacy M. Mervine AU - Evan A. Yost AU - Jonathan L. Sabo AU - Thomas R. Hynes AU - Catherine H. Berlot Y1 - 2006/07/01 UR - http://molpharm.aspetjournals.org/content/70/1/194.abstract N2 - The specificity of G protein βγ signaling demonstrated by in vivo knockouts is greater than expected based on in vitro assays of βγ function. In this study, we investigated the basis for this discrepancy by comparing the abilities of seven β1γ complexes containing γ1, γ2, γ5, γ7, γ10, γ11, or γ12 to interact with αs and of these γ subunits to compete for interaction with β1 in live human embryonic kidney (HEK) 293 cells. βγ complexes were imaged using bimolecular fluorescence complementation, in which fluorescence is produced by two nonfluorescent fragments (N and C) of cyan fluorescent protein (CFP) or yellow fluorescent protein (YFP) when brought together by proteins fused to each fragment. Plasma membrane targeting of αs-CFP varied inversely with its expression level, and the abilities of YFP-N-β1YFP-C-γ complexes to increase this targeting varied by 2-fold or less. However, there were larger differences in the abilities of the CFP-N-γ subunits to compete for association with CFP-C-β1. When the intensities of coexpressed CFP-C-β1CFP-N-γ (cyan) and CFP-C-β1YFP-N-γ2 (yellow) complexes were compared under conditions in which CFP-C-β1 was limiting, the CFP-N-γ subunits exhibited a 4.5-fold range in their abilities to compete with YFP-N-γ2 for association with CFP-C-β1. CFP-N-γ12 and CFP-N-γ1 were the strongest and weakest competitors, respectively. Taken together with previous demonstrations of a role for βγ in the specificity of receptor signaling, these results suggest that differences in the association preferences of coexpressed β and γ subunits for each other can determine which complexes predominate and participate in signaling pathways in intact cells. ER -